Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1-deleted adenoviruses.


Journal Article

We describe the use of a human bronchial xenograft model for studying the efficiency and biology of in vivo gene transfer into human bronchial epithelia with recombinant E1 deleted adenoviruses. All cell types in the surface epithelium except basal cells efficiently expressed the adenoviral transduced recombinant genes, lacZ and CFTR, for 3-5 weeks. Stable transgene expression was associated with high level expression of the early adenoviral gene, E2a, in a subset of transgene expressing cells and virtually undetectable expression of the late adenoviral genes encoding the structural proteins, hexon and fiber. These studies begin to address important issues that relate to safety and in vivo efficacy of recombinant adenoviruses for gene delivery into the human airway.

Full Text

Cited Authors

  • Engelhardt, JF; Yang, Y; Stratford-Perricaudet, LD; Allen, ED; Kozarsky, K; Perricaudet, M; Yankaskas, JR; Wilson, JM

Published Date

  • May 1993

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 27 - 34

PubMed ID

  • 7685651

Pubmed Central ID

  • 7685651

Electronic International Standard Serial Number (EISSN)

  • 1546-1718

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng0593-27


  • eng