SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development.
Published
Journal Article
The Drosophila ecdysone receptor (EcR)/ultraspiracle (USP) heterodimer is a key regulator in molting and metamorphoric processes, activating and repressing transcription in a sequence-specific manner. Here, we report the isolation of an EcR-interacting protein, SMRTER, which is structurally divergent but functionally similar to the vertebrate nuclear corepressors SMRT and N-CoR. SMRTER mediates repression by interacting with Sin3A, a repressor known to form a complex with the histone deacetylase Rpd3/HDAC. Importantly, we identify an EcR mutant allele that fails to bind SMRTER and is characterized by developmental defects and lethality. Together, these results reveal a novel nuclear receptor cofactor that exhibits evolutionary conservation in the mechanism to achieve repression and demonstrate the essential role of repression in hormone signaling.
Full Text
Duke Authors
Cited Authors
- Tsai, CC; Kao, HY; Yao, TP; McKeown, M; Evans, RM
Published Date
- August 1999
Published In
Volume / Issue
- 4 / 2
Start / End Page
- 175 - 186
PubMed ID
- 10488333
Pubmed Central ID
- 10488333
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/s1097-2765(00)80365-2
Language
- eng
Conference Location
- United States