SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development.

Published

Journal Article

The Drosophila ecdysone receptor (EcR)/ultraspiracle (USP) heterodimer is a key regulator in molting and metamorphoric processes, activating and repressing transcription in a sequence-specific manner. Here, we report the isolation of an EcR-interacting protein, SMRTER, which is structurally divergent but functionally similar to the vertebrate nuclear corepressors SMRT and N-CoR. SMRTER mediates repression by interacting with Sin3A, a repressor known to form a complex with the histone deacetylase Rpd3/HDAC. Importantly, we identify an EcR mutant allele that fails to bind SMRTER and is characterized by developmental defects and lethality. Together, these results reveal a novel nuclear receptor cofactor that exhibits evolutionary conservation in the mechanism to achieve repression and demonstrate the essential role of repression in hormone signaling.

Full Text

Duke Authors

Cited Authors

  • Tsai, CC; Kao, HY; Yao, TP; McKeown, M; Evans, RM

Published Date

  • August 1999

Published In

Volume / Issue

  • 4 / 2

Start / End Page

  • 175 - 186

PubMed ID

  • 10488333

Pubmed Central ID

  • 10488333

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(00)80365-2

Language

  • eng

Conference Location

  • United States