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Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation.

Publication ,  Journal Article
Kawasaki, H; Eckner, R; Yao, TP; Taira, K; Chiu, R; Livingston, DM; Yokoyama, KK
Published in: Nature
May 21, 1998

The related proteins p300 and CBP (cAMP-response-element-binding protein (CREB)-binding protein)) are transcriptional co-activators that act with other factors to regulate gene expression and play roles in many cell-differentiation and signal transduction pathways. Both proteins have intrinsic histone-acetyltransferase activity and may act directly on chromatin, of which histone is a component, to facilitate transcription. They are also involved in growth control pathways, as shown by their interaction with the tumour suppressor p53 and the viral oncogenes E1A and SV40 T antigen. Here we report functional differences of p300 and CBP in vivo. We examined their roles during retinoic-acid-induced differentiation, cell-cycle exit and programmed cell death (apoptosis) of embryonal carcinoma F9 cells, using hammerhead ribozymes capable of cleaving either p300 or CBP messenger RNAs. F9 cells expressing a p300-specific ribozyme became resistant to retinoic-acid-induced differentiation, whereas cells expressing a CBP-specific ribozyme were unaffected. Similarly, retinoic-acid-induced transcriptional upregulation of the cell-cycle inhibitor p21Cip1 required normal levels of p300, but not CBP, whereas the reverse was true for p27Kip1. In contrast, both ribozymes blocked retinoic-acid-induced apoptosis, indicating that both co-activators are required for this process. Thus, despite their similarities, p300 and CBP have distinct functions during retinoic-acid-induced differentiation of F9 cells.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

May 21, 1998

Volume

393

Issue

6682

Start / End Page

284 / 289

Location

England

Related Subject Headings

  • Tumor Suppressor Proteins
  • Tumor Cells, Cultured
  • Tretinoin
  • Transcription Factors
  • Trans-Activators
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • RNA, Catalytic
  • Nuclear Proteins
  • Microtubule-Associated Proteins
 

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Kawasaki, H., Eckner, R., Yao, T. P., Taira, K., Chiu, R., Livingston, D. M., & Yokoyama, K. K. (1998). Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation. Nature, 393(6682), 284–289. https://doi.org/10.1038/30538
Kawasaki, H., R. Eckner, T. P. Yao, K. Taira, R. Chiu, D. M. Livingston, and K. K. Yokoyama. “Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation.Nature 393, no. 6682 (May 21, 1998): 284–89. https://doi.org/10.1038/30538.
Kawasaki H, Eckner R, Yao TP, Taira K, Chiu R, Livingston DM, et al. Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation. Nature. 1998 May 21;393(6682):284–9.
Kawasaki, H., et al. “Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation.Nature, vol. 393, no. 6682, May 1998, pp. 284–89. Pubmed, doi:10.1038/30538.
Kawasaki H, Eckner R, Yao TP, Taira K, Chiu R, Livingston DM, Yokoyama KK. Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation. Nature. 1998 May 21;393(6682):284–289.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

May 21, 1998

Volume

393

Issue

6682

Start / End Page

284 / 289

Location

England

Related Subject Headings

  • Tumor Suppressor Proteins
  • Tumor Cells, Cultured
  • Tretinoin
  • Transcription Factors
  • Trans-Activators
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • RNA, Catalytic
  • Nuclear Proteins
  • Microtubule-Associated Proteins