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Ecdysone-inducible gene expression in mammalian cells and transgenic mice.

Publication ,  Journal Article
No, D; Yao, TP; Evans, RM
Published in: Proc Natl Acad Sci U S A
April 16, 1996

During metamorphosis of Drosophila melanogaster, a cascade of morphological changes is triggered by the steroid hormone 20-OH ecdysone via the ecdysone receptor, a member of the nuclear receptor superfamily. In this report, we have transferred insect hormone responsiveness to mammalian cells by the stable expression of a modified ecdysone receptor that regulates an optimized ecdysone responsive promoter. Inductions reaching 4 orders of magnitude have been achieved upon treatment with hormone. Transgenic mice expressing the modified ecdysone receptor can activate an integrated ecdysone responsive promoter upon administration of hormone. A comparison of tetracycline-based and ecdysone-based inducible systems reveals the ecdysone regulatory system exhibits lower basal activity and higher inducibility. Since ecdysone administration has no apparent effect on mammals, its use for regulating genes should be excellent for transient inducible expression of any gene in transgenic mice and for gene therapy.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 16, 1996

Volume

93

Issue

8

Start / End Page

3346 / 3351

Location

United States

Related Subject Headings

  • Transfection
  • Tetracycline
  • Receptors, Steroid
  • Promoter Regions, Genetic
  • Oligodeoxyribonucleotides
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Gene Expression
 

Citation

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No, D., Yao, T. P., & Evans, R. M. (1996). Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A, 93(8), 3346–3351. https://doi.org/10.1073/pnas.93.8.3346
No, D., T. P. Yao, and R. M. Evans. “Ecdysone-inducible gene expression in mammalian cells and transgenic mice.Proc Natl Acad Sci U S A 93, no. 8 (April 16, 1996): 3346–51. https://doi.org/10.1073/pnas.93.8.3346.
No D, Yao TP, Evans RM. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3346–51.
No, D., et al. “Ecdysone-inducible gene expression in mammalian cells and transgenic mice.Proc Natl Acad Sci U S A, vol. 93, no. 8, Apr. 1996, pp. 3346–51. Pubmed, doi:10.1073/pnas.93.8.3346.
No D, Yao TP, Evans RM. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3346–3351.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 16, 1996

Volume

93

Issue

8

Start / End Page

3346 / 3351

Location

United States

Related Subject Headings

  • Transfection
  • Tetracycline
  • Receptors, Steroid
  • Promoter Regions, Genetic
  • Oligodeoxyribonucleotides
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Gene Expression