Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response.

Journal Article (Journal Article)

In a search for retinoid X receptor-like molecules in Drosophila, we have identified an additional member of the nuclear receptor superfamily, XR78E/F. In the DNA-binding domain, XR78E/F is closely related to the mammalian receptor TR2, as well as to the nuclear receptors Coup-TF and Seven-up. We demonstrate that XR78E/F binds as a homodimer to direct repeats of the sequence AGGTCA. In transient transfection assays, XR78E/F represses ecdysone signaling in a DNA-binding-dependent fashion. XR78E/F has its highest expression in third-instar larvae and prepupae. These experiments suggest that XR78E/F may play a regulatory role in the transcriptional cascade triggered by the hormone ecdysone in Drosophila.

Full Text

Duke Authors

Cited Authors

  • Zelhof, AC; Yao, TP; Evans, RM; McKeown, M

Published Date

  • November 7, 1995

Published In

Volume / Issue

  • 92 / 23

Start / End Page

  • 10477 - 10481

PubMed ID

  • 7479823

Pubmed Central ID

  • PMC40634

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.92.23.10477


  • eng

Conference Location

  • United States