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Functional ecdysone receptor is the product of EcR and Ultraspiracle genes.

Publication ,  Journal Article
Yao, TP; Forman, BM; Jiang, Z; Cherbas, L; Chen, JD; McKeown, M; Cherbas, P; Evans, RM
Published in: Nature
December 2, 1993

Although the biological activity of the insect moulting hormone ecdysone, is manifested through a hormonally regulated transcriptional cascade associated with chromosomal puffing, a direct association of the receptor with the puff has yet to be established. The cloned ecdysone receptor (EcR) is by itself incapable of high-affinity DNA binding or transcriptional activation. Rather, these activities are dependent on heterodimer formation with Ultraspiracle (USP) the insect homologue of vertebrate retinoid X receptor. Here we report that native EcR and USP are co-localized on ecdysone-responsive loci of polytene chromosomes. Moreover, we show that natural ecdysones selectively promote physical association between EcR and USP, and conversely, that high-affinity hormone binding requires both EcR and USP. Replacement of USP with retinoid X receptor produces heterodimers with distinct pharmacological and functional properties. These results redefine the ecdysone receptor as a dynamic complex whose activity may be altered by combinatorial interactions among subunits and ligand.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 2, 1993

Volume

366

Issue

6454

Start / End Page

476 / 479

Location

England

Related Subject Headings

  • Transcription Factors
  • Retinoid X Receptors
  • Receptors, Steroid
  • Receptors, Retinoic Acid
  • Receptors, Cytoplasmic and Nuclear
  • Genes, Insect
  • General Science & Technology
  • Ecdysterone
  • Ecdysone
  • Drosophila Proteins
 

Citation

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Yao, T. P., Forman, B. M., Jiang, Z., Cherbas, L., Chen, J. D., McKeown, M., … Evans, R. M. (1993). Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature, 366(6454), 476–479. https://doi.org/10.1038/366476a0
Yao, T. P., B. M. Forman, Z. Jiang, L. Cherbas, J. D. Chen, M. McKeown, P. Cherbas, and R. M. Evans. “Functional ecdysone receptor is the product of EcR and Ultraspiracle genes.Nature 366, no. 6454 (December 2, 1993): 476–79. https://doi.org/10.1038/366476a0.
Yao TP, Forman BM, Jiang Z, Cherbas L, Chen JD, McKeown M, et al. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature. 1993 Dec 2;366(6454):476–9.
Yao, T. P., et al. “Functional ecdysone receptor is the product of EcR and Ultraspiracle genes.Nature, vol. 366, no. 6454, Dec. 1993, pp. 476–79. Pubmed, doi:10.1038/366476a0.
Yao TP, Forman BM, Jiang Z, Cherbas L, Chen JD, McKeown M, Cherbas P, Evans RM. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature. 1993 Dec 2;366(6454):476–479.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 2, 1993

Volume

366

Issue

6454

Start / End Page

476 / 479

Location

England

Related Subject Headings

  • Transcription Factors
  • Retinoid X Receptors
  • Receptors, Steroid
  • Receptors, Retinoic Acid
  • Receptors, Cytoplasmic and Nuclear
  • Genes, Insect
  • General Science & Technology
  • Ecdysterone
  • Ecdysone
  • Drosophila Proteins