High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.

Published

Journal Article

A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

Full Text

Duke Authors

Cited Authors

  • Polyak, B; Fishbein, I; Chorny, M; Alferiev, I; Williams, D; Yellen, B; Friedman, G; Levy, RJ

Published Date

  • January 8, 2008

Published In

Volume / Issue

  • 105 / 2

Start / End Page

  • 698 - 703

PubMed ID

  • 18182491

Pubmed Central ID

  • 18182491

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0708338105

Language

  • eng