High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.
Published
Journal Article
A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.
Full Text
Duke Authors
Cited Authors
- Polyak, B; Fishbein, I; Chorny, M; Alferiev, I; Williams, D; Yellen, B; Friedman, G; Levy, RJ
Published Date
- January 8, 2008
Published In
Volume / Issue
- 105 / 2
Start / End Page
- 698 - 703
PubMed ID
- 18182491
Pubmed Central ID
- 18182491
Electronic International Standard Serial Number (EISSN)
- 1091-6490
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.0708338105
Language
- eng