Retention of pendrin in the endoplasmic reticulum is a major mechanism for Pendred syndrome.

Journal Article (Journal Article)

Pendred syndrome is a major cause of congenital deafness, goiter and defective iodide organification. Mutations in the transmembrane protein, pendrin, cause diminished export of iodide from thyroid follicular cells to the colloid and are associated with the syndrome. We used green fluorescent protein (GFP) chimeras of wild-type (WT) pendrin and three common natural mutants (L236P, T416P and G384) to study their intracellular trafficking in living cells. Time-lapse imaging, dual color labeling and fluorescent recovery after photobleaching (FRAP) studies demonstrated that GFP-WT pendrin targets to the plasma membrane. In contrast, all three mutant pendrins were retained in the endoplasmic reticulum (ER) in co-localization studies with ER and Golgi markers. The ER retention of L236P appeared to be selective as this mutant did not prevent a viral membrane protein, VSVGtsO45 or wild-type pendrin from targeting the plasma membrane. These findings suggest that ER retention and defective plasma membrane targeting of pendrin mutants play a key role in the pathogenesis of Pendred syndrome.

Full Text

Duke Authors

Cited Authors

  • Rotman-Pikielny, P; Hirschberg, K; Maruvada, P; Suzuki, K; Royaux, IE; Green, ED; Kohn, LD; Lippincott-Schwartz, J; Yen, PM

Published Date

  • October 1, 2002

Published In

Volume / Issue

  • 11 / 21

Start / End Page

  • 2625 - 2633

PubMed ID

  • 12354788

International Standard Serial Number (ISSN)

  • 0964-6906

Digital Object Identifier (DOI)

  • 10.1093/hmg/11.21.2625


  • eng

Conference Location

  • England