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ROR alpha augments thyroid hormone receptor-mediated transcriptional activation.

Publication ,  Journal Article
Koibuchi, N; Liu, Y; Fukuda, H; Takeshita, A; Yen, PM; Chin, WW
Published in: Endocrinology
March 1999

This study is designed to clarify the role of an orphan nuclear hormone receptor, ROR alpha, on thyroid hormone (TH) receptor (TR)-mediated transcription on a TH-response element (TRE). A transient transfection study using various TREs [i.e., F2 (chick lysozyme TRE), DR4 (direct repeat), and palindrome TRE] and TR and ROR alpha1 was performed. When ROR alpha1 and TR were cotransfected into CV1 cells, ROR alpha1 enhanced the transactivation by liganded-TR on all TREs tested without an effect on basal repression by unliganded TR. By electrophoretic mobility shift assay, on the other hand, although ROR alpha bound to all TREs tested as a monomer, no (or weak) TR and ROR alpha1 heterodimer formation was observed on various TREs except when a putative ROR-response element was present. The transactivation by ROR alpha1 on a ROR-response element, which does not contain a TRE, was not enhanced by TR. The effect of ROR alpha1 on the TREs is unique, because, whereas other nuclear hormone receptors (such as vitamin D receptor) may competitively bind to TRE to exert dominant negative function, ROR alpha1 augmented TR action. These results indicate that ROR alpha1 may modify the effect of liganded TR on TH-responsive genes. Because TR and ROR alpha are coexpressed in cerebellar Purkinje cells, and perinatal hypothyroid animals and ROR alpha-disrupted animals show similar abnormalities of this cell type, cross-talk between these two receptors may play a critical role in Purkinje cell differentiation.

Duke Scholars

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

March 1999

Volume

140

Issue

3

Start / End Page

1356 / 1364

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Trans-Activators
  • Thyrotropin-Releasing Hormone
  • Sequence Analysis, DNA
  • Response Elements
  • Receptors, Cytoplasmic and Nuclear
  • Rats
  • Nuclear Receptor Subfamily 1, Group F, Member 1
  • Nerve Tissue Proteins
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Koibuchi, N., Liu, Y., Fukuda, H., Takeshita, A., Yen, P. M., & Chin, W. W. (1999). ROR alpha augments thyroid hormone receptor-mediated transcriptional activation. Endocrinology, 140(3), 1356–1364. https://doi.org/10.1210/endo.140.3.6562
Koibuchi, N., Y. Liu, H. Fukuda, A. Takeshita, P. M. Yen, and W. W. Chin. “ROR alpha augments thyroid hormone receptor-mediated transcriptional activation.Endocrinology 140, no. 3 (March 1999): 1356–64. https://doi.org/10.1210/endo.140.3.6562.
Koibuchi N, Liu Y, Fukuda H, Takeshita A, Yen PM, Chin WW. ROR alpha augments thyroid hormone receptor-mediated transcriptional activation. Endocrinology. 1999 Mar;140(3):1356–64.
Koibuchi, N., et al. “ROR alpha augments thyroid hormone receptor-mediated transcriptional activation.Endocrinology, vol. 140, no. 3, Mar. 1999, pp. 1356–64. Pubmed, doi:10.1210/endo.140.3.6562.
Koibuchi N, Liu Y, Fukuda H, Takeshita A, Yen PM, Chin WW. ROR alpha augments thyroid hormone receptor-mediated transcriptional activation. Endocrinology. 1999 Mar;140(3):1356–1364.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

March 1999

Volume

140

Issue

3

Start / End Page

1356 / 1364

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Trans-Activators
  • Thyrotropin-Releasing Hormone
  • Sequence Analysis, DNA
  • Response Elements
  • Receptors, Cytoplasmic and Nuclear
  • Rats
  • Nuclear Receptor Subfamily 1, Group F, Member 1
  • Nerve Tissue Proteins
  • Mice