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Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells

Publication ,  Journal Article
Lin, HY; Davis, FB; Yen, PM; Davis, PJ
Published in: Journal of Investigative Medicine
January 1, 1996

Milrinone (Mil) is a bipyridine inotropic agent which has structural homologies with thyroid hormone and thyromimetic effects in several tissues. We have examined the action of Mil and amrinone (Am) in a system in which Lthyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) enhance the antiviral action of recombinant human interferon-y (IFN-y) in cultured cells. Mil or Am (107-10"5 M) were added to HeLa cells either for 24 h prior to 24 h of IFN-y, or together with IFN-y for 24 h. Antiviral effect was then assayed. Mil and Am alone had no antiviral effect. Mil (107 M) enhanced IFN-ys action only when added prior to IFN-y whereas Mil (105 M) also enhanced IFN-y action during coincubation with IFN-y. Am was ineffective. The effect of Mil was blocked by cycloheximidQ (25 ng/ml) when both agents were applied prior to IFN-y treatment. Mil action was not blocked by tetraiodothyroacetic acid (tetrac) or triiodothyroacetic acid (triac), in contrast to prior studies with T4 and these analogues in this assay. In studies of T3-binding to the receptor TR, Mil did not displace T3. In cells cotransfected with TRa or TR expression plasmid, Mil neither transactivated, nor blocked, T3-mediated transcriptional activation. Thus, milrinone mimicked thyroid hormone's potentiation of IFN-ys antiviral effect in a protein synthesis-dependent manner, but the bipyridine's action was not mediated by the thyroid hormone receptor.

Duke Scholars

Published In

Journal of Investigative Medicine

ISSN

1708-8267

Publication Date

January 1, 1996

Volume

44

Issue

3

Related Subject Headings

  • General Clinical Medicine
  • 1103 Clinical Sciences
 

Citation

APA
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ICMJE
MLA
NLM
Lin, H. Y., Davis, F. B., Yen, P. M., & Davis, P. J. (1996). Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells. Journal of Investigative Medicine, 44(3).
Lin, H. Y., F. B. Davis, P. M. Yen, and P. J. Davis. “Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells.” Journal of Investigative Medicine 44, no. 3 (January 1, 1996).
Lin HY, Davis FB, Yen PM, Davis PJ. Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells. Journal of Investigative Medicine. 1996 Jan 1;44(3).
Lin, H. Y., et al. “Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells.” Journal of Investigative Medicine, vol. 44, no. 3, Jan. 1996.
Lin HY, Davis FB, Yen PM, Davis PJ. Milrinone mimics thyroid hormone potentiation of the antiviral activity of human interferon-γ in hela cells. Journal of Investigative Medicine. 1996 Jan 1;44(3).

Published In

Journal of Investigative Medicine

ISSN

1708-8267

Publication Date

January 1, 1996

Volume

44

Issue

3

Related Subject Headings

  • General Clinical Medicine
  • 1103 Clinical Sciences