Skip to main content
Journal cover image

The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts.

Publication ,  Journal Article
Allain, TJ; Yen, PM; Flanagan, AM; McGregor, AM
Published in: Eur J Clin Invest
May 1996

Hyperthyroidism is associated with an increase in both osteoblast and osteoclast activity. We have previously shown that, in vitro, osteoclasts do not respond directly to tri-iodothyronine to increase bone resorption but that the effect is mediated by another bone cell, probably the osteoblast. To investigate this issue further we have studied the isoform-specific expression of thyroid receptor (TR) protein in human osteoclasts derived from an osteoclastoma (giant cell tumour of bone) and the expression of TR mRNA and protein in the osteoblastic cell lines MG 63 and UMR 106. Three major TR receptor variants have been described; TR alpha 1 and TR beta are functional receptors whereas c-erbA alpha 2 is a non-functional variant. Northern blot analysis using [32P]-cDNA probes against human TR alpha 1, c-erbA alpha 2 and TR beta demonstrated specific binding of these probes to mRNA from MG 63 and UMR 106. mRNA for all three receptor variants was observed in both cell lines, in UMR 106 multiple mRNA transcripts were present for TR alpha 1 and TR beta. Immunohistochemical staining with antibodies recognizing a common TR alpha epitope and specific c-erbA alpha 2 and TR beta epitopes extended these observations by demonstrating receptor protein in both osteoblasts and osteoclasts. These findings are consistent with previous observations of TR expression in osteoblast-like-cells and are the first direct demonstration of TR in osteoclasts.

Duke Scholars

Published In

Eur J Clin Invest

DOI

ISSN

0014-2972

Publication Date

May 1996

Volume

26

Issue

5

Start / End Page

418 / 425

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Thyroid Gland
  • Receptors, Thyroid Hormone
  • Rats
  • RNA, Ribosomal
  • RNA, Messenger
  • Osteoclasts
  • Osteoblasts
  • Humans
  • Giant Cell Tumor of Bone
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Allain, T. J., Yen, P. M., Flanagan, A. M., & McGregor, A. M. (1996). The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts. Eur J Clin Invest, 26(5), 418–425. https://doi.org/10.1046/j.1365-2362.1996.160289.x
Allain, T. J., P. M. Yen, A. M. Flanagan, and A. M. McGregor. “The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts.Eur J Clin Invest 26, no. 5 (May 1996): 418–25. https://doi.org/10.1046/j.1365-2362.1996.160289.x.
Allain TJ, Yen PM, Flanagan AM, McGregor AM. The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts. Eur J Clin Invest. 1996 May;26(5):418–25.
Allain, T. J., et al. “The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts.Eur J Clin Invest, vol. 26, no. 5, May 1996, pp. 418–25. Pubmed, doi:10.1046/j.1365-2362.1996.160289.x.
Allain TJ, Yen PM, Flanagan AM, McGregor AM. The isoform-specific expression of the tri-iodothyronine receptor in osteoblasts and osteoclasts. Eur J Clin Invest. 1996 May;26(5):418–425.
Journal cover image

Published In

Eur J Clin Invest

DOI

ISSN

0014-2972

Publication Date

May 1996

Volume

26

Issue

5

Start / End Page

418 / 425

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Thyroid Gland
  • Receptors, Thyroid Hormone
  • Rats
  • RNA, Ribosomal
  • RNA, Messenger
  • Osteoclasts
  • Osteoblasts
  • Humans
  • Giant Cell Tumor of Bone