Steroid hormone receptors selectively affect transcriptional activation but not basal repression by thyroid hormone receptors.

Published

Journal Article

Thyroid hormone receptors (TRs) and steroid hormone receptors belong to a large superfamily of nuclear hormone receptors. The interactions between these receptor subfamilies are poorly understood. In this study, cotransfection assays were used to examine the effects of estrogen and glucocorticoid receptors on TR-mediated repression of basal transcription by unliganded TR and transcriptional activation by liganded TR with two different thyroid hormone response element-containing reporter plasmids. Surprisingly, it was found that steroid hormone receptors blocked T3-mediated transcriptional activation with little or no effect on basal repression by unliganded TR. The mechanism for blocking TR-mediated transcriptional activation does not require steroid hormone receptor binding to the thyroid hormone response element but, rather, may involve titration of a critical coactivator(s) required for T3-mediated transcriptional activation. These studies strongly suggest divergent pathways for transcriptional activation and basal repression by TRs. Additionally, these studies raise the potential for nuclear hormone receptors to modulate TR-mediated transcriptional activation in steroid hormone-responsive tissues.

Full Text

Duke Authors

Cited Authors

  • Yen, PM; Wilcox, EC; Chin, WW

Published Date

  • February 1995

Published In

Volume / Issue

  • 136 / 2

Start / End Page

  • 440 - 445

PubMed ID

  • 7835274

Pubmed Central ID

  • 7835274

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo.136.2.7835274

Language

  • eng

Conference Location

  • United States