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Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.

Publication ,  Journal Article
Kudo, F; Yamamoto, Y; Yokoyama, K; Eguchi, T; Kakinuma, K
Published in: J Antibiot (Tokyo)
December 2005
Published version (DOI) Link to item

NeoA, B, and C encoded in the neomycin biosynthetic gene cluster have been enzymatically confirmed to be responsible to the formation of 2-deoxystreptamine (DOS) in Streptomyces fradiae. NeoC was functionally characterized as 2-deoxy-scyllo-inosose synthase, which catalyzes the carbocycle formation from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose. Further, NeoA appeared to catalyze the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(P)+ forming 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI). Consequently, NeoA was characterized as 2-deoxy-scyllo-inosamine dehydrogenase. Finally, amino-DOI produced by NeoA from DOIA was transformed into DOS by NeoB. Since NeoB (Neo6) was also reported to be L-glutamine:2-deoxy-scyllo-inosose aminotransferase, all the enzymes in the DOS biosynthesis were characterized for the first time.

Duke Scholars

Kenichi Yokoyama
Author Kenichi Yokoyama Biochemistry
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Published In

J Antibiot (Tokyo)

DOI

10.1038/ja.2005.104

ISSN

0021-8820

Publication Date

December 2005

Volume

58

Issue

12

Start / End Page

766 / 774

Location

England

Related Subject Headings

  • Streptomyces
  • Multigene Family
  • Molecular Sequence Data
  • Microbiology
  • Medicinal & Biomolecular Chemistry
  • Mass Spectrometry
  • Hexosamines
  • Chromatography, Liquid
  • Chromatography, High Pressure Liquid
  • Carbohydrate Sequence
 

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Kudo, F., Yamamoto, Y., Yokoyama, K., Eguchi, T., & Kakinuma, K. (2005). Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. J Antibiot (Tokyo), 58(12), 766–774. https://doi.org/10.1038/ja.2005.104
Kudo, Fumitaka, Yasuhito Yamamoto, Kenichi Yokoyama, Tadashi Eguchi, and Katsumi Kakinuma. “Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.” J Antibiot (Tokyo) 58, no. 12 (December 2005): 766–74. https://doi.org/10.1038/ja.2005.104.
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. J Antibiot (Tokyo). 2005 Dec;58(12):766–74.
Kudo, Fumitaka, et al. “Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.” J Antibiot (Tokyo), vol. 58, no. 12, Dec. 2005, pp. 766–74. Pubmed, doi:10.1038/ja.2005.104.
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. J Antibiot (Tokyo). 2005 Dec;58(12):766–774.

Published In

J Antibiot (Tokyo)

DOI

10.1038/ja.2005.104

ISSN

0021-8820

Publication Date

December 2005

Volume

58

Issue

12

Start / End Page

766 / 774

Location

England

Related Subject Headings

  • Streptomyces
  • Multigene Family
  • Molecular Sequence Data
  • Microbiology
  • Medicinal & Biomolecular Chemistry
  • Mass Spectrometry
  • Hexosamines
  • Chromatography, Liquid
  • Chromatography, High Pressure Liquid
  • Carbohydrate Sequence