Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in Mammalian cells.

Journal Article (Journal Article)

Lipid-derived inositol phosphates (InsPs) comprise a family of second messengers that arise through the action of six classes of InsP kinases, generally referred to as IPKs. Genetic studies have indicated that InsPs play critical roles in embryonic development, but the mechanisms of action for InsPs in mammalian cellular function are largely unknown. This chapter outlines a method for manipulating cellular InsP profiles through the coexpression of a constitutively active G protein and various IPKs. It provides a mechanism by which the metabolism of a variety of InsPs can be upregulated, enabling the evaluation of the effects of these InsPs on cellular functions.

Full Text

Duke Authors

Cited Authors

  • Otto, JC; York, JD

Published Date

  • 2010

Published In

Volume / Issue

  • 645 /

Start / End Page

  • 47 - 60

PubMed ID

  • 20645180

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-60327-175-2_3


  • eng

Conference Location

  • United States