Biochemical analysis of inositol phosphate kinases.

Journal Article

Lipid-derived inositol phosphates (IPs) are a complex group of second messengers generated by the sequential phosphorylation of inositol 1,4,5-trisphosphate (IP(3)). Synthetic pathways leading from IP(3) to the formation of inositol tetrakisphosphate IP(4), inositol pentakisphosphate IP(5), inositol hexakisphosphate IP(6), and inositol pyrophosphates PP-IPs have been elucidated in eukaryotes from yeast to human. Studies have attributed a variety of cellular functions to IPs, highlighting the importance of understanding how the pathways for their synthesis are regulated. This chapter summarizes experimental techniques for the biochemical characterization of the key inositol phosphate kinases IPKs necessary for producing the diverse array of IP species.

Full Text

Duke Authors

Cited Authors

  • Otto, JC; Mulugu, S; Fridy, PC; Chiou, S-T; Armbruster, BN; Ribeiro, AA; York, JD

Published Date

  • 2007

Published In

Volume / Issue

  • 434 /

Start / End Page

  • 171 - 185

PubMed ID

  • 17954248

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(07)34010-X

Language

  • eng

Conference Location

  • United States