Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases.

Journal Article (Journal Article)

Phytate (inositol hexakisphosphate, IP6) is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2beta, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate (IP5) 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.

Full Text

Duke Authors

Cited Authors

  • Stevenson-Paulik, J; Bastidas, RJ; Chiou, S-T; Frye, RA; York, JD

Published Date

  • August 30, 2005

Published In

Volume / Issue

  • 102 / 35

Start / End Page

  • 12612 - 12617

PubMed ID

  • 16107538

Pubmed Central ID

  • PMC1194928

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0504172102


  • eng

Conference Location

  • United States