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Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy.

Publication ,  Journal Article
Rivas, MP; Kearns, BG; Xie, Z; Guo, S; Sekar, MC; Hosaka, K; Kagiwada, S; York, JD; Bankaitis, VA
Published in: Mol Biol Cell
July 1999

SacIp dysfunction results in bypass of the requirement for phosphatidylinositol transfer protein (Sec14p) function in yeast Golgi processes. This effect is accompanied by alterations in inositol phospholipid metabolism and inositol auxotrophy. Elucidation of how sac1 mutants effect "bypass Sec14p" will provide insights into Sec14p function in vivo. We now report that, in addition to a dramatic accumulation of phosphatidylinositol-4-phosphate, sac1 mutants also exhibit a specific acceleration of phosphatidylcholine biosynthesis via the CDP-choline pathway. This phosphatidylcholine metabolic phenotype is sensitive to the two physiological challenges that abolish bypass Sec14p in sac1 strains; i.e. phospholipase D inactivation and expression of bacterial diacylglycerol (DAG) kinase. Moreover, we demonstrate that accumulation of phosphatidylinositol-4-phosphate in sac1 mutants is insufficient to effect bypass Sec14p. These data support a model in which phospholipase D activity contributes to generation of DAG that, in turn, effects bypass Sec14p. A significant fate for this DAG is consumption by the CDP-choline pathway. Finally, we determine that CDP-choline pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does not involve obvious defects in transcriptional expression of the INO1 gene.

Duke Scholars

Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

July 1999

Volume

10

Issue

7

Start / End Page

2235 / 2250

Location

United States

Related Subject Headings

  • Yeasts
  • Saccharomyces cerevisiae Proteins
  • Phospholipid Transfer Proteins
  • Phospholipase D
  • Phosphatidylinositol Phosphates
  • Phosphatidylcholines
  • Mutation
  • Membrane Transport Proteins
  • Membrane Proteins
  • Lipid Metabolism
 

Citation

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Rivas, M. P., Kearns, B. G., Xie, Z., Guo, S., Sekar, M. C., Hosaka, K., … Bankaitis, V. A. (1999). Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy. Mol Biol Cell, 10(7), 2235–2250. https://doi.org/10.1091/mbc.10.7.2235
Rivas, M. P., B. G. Kearns, Z. Xie, S. Guo, M. C. Sekar, K. Hosaka, S. Kagiwada, J. D. York, and V. A. Bankaitis. “Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy.Mol Biol Cell 10, no. 7 (July 1999): 2235–50. https://doi.org/10.1091/mbc.10.7.2235.
Rivas MP, Kearns BG, Xie Z, Guo S, Sekar MC, Hosaka K, et al. Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy. Mol Biol Cell. 1999 Jul;10(7):2235–50.
Rivas, M. P., et al. “Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy.Mol Biol Cell, vol. 10, no. 7, July 1999, pp. 2235–50. Pubmed, doi:10.1091/mbc.10.7.2235.
Rivas MP, Kearns BG, Xie Z, Guo S, Sekar MC, Hosaka K, Kagiwada S, York JD, Bankaitis VA. Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy. Mol Biol Cell. 1999 Jul;10(7):2235–2250.

Published In

Mol Biol Cell

DOI

ISSN

1059-1524

Publication Date

July 1999

Volume

10

Issue

7

Start / End Page

2235 / 2250

Location

United States

Related Subject Headings

  • Yeasts
  • Saccharomyces cerevisiae Proteins
  • Phospholipid Transfer Proteins
  • Phospholipase D
  • Phosphatidylinositol Phosphates
  • Phosphatidylcholines
  • Mutation
  • Membrane Transport Proteins
  • Membrane Proteins
  • Lipid Metabolism