Skip to main content

Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase?

Publication ,  Journal Article
Malik, N; Poltoratsky, VP; York, JD; Lieber, MR; Carter, TH
Published in: FASEB Journal
December 1, 1996

Phosphorylation of macromolecules such as proteins and lipids regulates diverse cellular processes, including DNA repair and the cell cycle. Until recently, lipid and protein kinase activities were considered to be separate biochemical entities. However several laboratories have reported potential "dual function" kinases. DNA-activated protein kinase is a serine/threonine protein kinase that interacts with a DNA end-binding heterodimeric protein, Ku activated by double-stranded DNA. Genomic clones that contain the DNA-PK gene complement the murine scid defect, indicating that DNA-PK affects double-strand (ds) break repair and V(D)J recombination. Here we describe the cDNA sequence of the region that corresponds to about 100 kDa of C-terminal sequence of this large protein. This region contains a kinase domain that has strong homology to phosphatidylinositol kinases. We also find PI-4 kinase activity in some highly purified preparations of DNA-PKcs, although this is not always the case. Cryptic PI-4 kinase activity of DNA-PK could be revealed either by post translational modification of the DNA-PKcs, for example by phosphorylation or partial proteolysis of DNA-PKcs, or by association with regulatory protein(s). Fractionation of HeLa nuclear extract on sequence specific DNA affinity column reveals three major polypeptides in addition to DNA-PKcs. in fractions with peak protein kinase activity. None of these polypeptides are Ku-related. Further analysis of these proteins involving their possible role in the regulation of DNA-PKcs is underway.

Duke Scholars

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1996

Volume

10

Issue

6

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Malik, N., Poltoratsky, V. P., York, J. D., Lieber, M. R., & Carter, T. H. (1996). Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase? FASEB Journal, 10(6).
Malik, N., V. P. Poltoratsky, J. D. York, M. R. Lieber, and T. H. Carter. “Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase?FASEB Journal 10, no. 6 (December 1, 1996).
Malik N, Poltoratsky VP, York JD, Lieber MR, Carter TH. Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase? FASEB Journal. 1996 Dec 1;10(6).
Malik, N., et al. “Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase?FASEB Journal, vol. 10, no. 6, Dec. 1996.
Malik N, Poltoratsky VP, York JD, Lieber MR, Carter TH. Is dna dependent protein kinase, dna-pk, a dual function (phospholipid/protein) kinase? FASEB Journal. 1996 Dec 1;10(6).

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1996

Volume

10

Issue

6

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology