Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.
Published
Journal Article
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.
Full Text
Duke Authors
Cited Authors
- Balagaddé, FK; You, L; Hansen, CL; Arnold, FH; Quake, SR
Published Date
- July 2005
Published In
Volume / Issue
- 309 / 5731
Start / End Page
- 137 - 140
PubMed ID
- 15994559
Pubmed Central ID
- 15994559
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.1109173
Language
- eng