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Requirement of transforming growth factor-beta (TGF-beta) type II receptor for TGF-beta-induced proliferation and growth inhibition.

Publication ,  Journal Article
Zhao, Y; Young, SL
Published in: J Biol Chem
February 2, 1996

Growth regulation of fibroblasts is important for lung development and repair of lung injury. In this study, we investigated the role of transforming growth factor-beta (TGF-beta) type II receptor in the TGF-beta-dependent proliferative response of lung fibroblasts. TGF-beta stimulated the proliferation of adult lung fibroblasts at a low concentration (1 ng/ml), but inhibited the growth of fetal lung fibroblasts in a dose-dependent fashion (0.1-10 ng/ml). Cross-linking and Northern analysis demonstrated that the two lung fibroblast cell lines expressed the TGF-beta type I receptor (T beta RI) and type II receptor (T beta RII). We overexpressed in lung fibroblasts a truncated derivative of T beta RII that lacked the cytoplasmic serine/threonine kinase domain (T beta RII delta K). T beta RII delta K was a dominant-negative inhibitor of TGF-beta signal transduction blocking not only TGF-beta-induced mitogenic action upon adult lung fibroblasts but also TGF-beta-induced growth inhibition of fetal lung fibroblasts. The results indicate that the type II receptor is indispensable for mediating both the mitogenic and antiproliferative effects of TGF-beta upon lung fibroblasts.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 2, 1996

Volume

271

Issue

5

Start / End Page

2369 / 2372

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Receptors, Transforming Growth Factor beta
  • Rats
  • Molecular Sequence Data
  • Lung
  • DNA Primers
  • Cells, Cultured
  • Cell Division
  • Biochemistry & Molecular Biology
  • Base Sequence
 

Citation

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Zhao, Y., & Young, S. L. (1996). Requirement of transforming growth factor-beta (TGF-beta) type II receptor for TGF-beta-induced proliferation and growth inhibition. J Biol Chem, 271(5), 2369–2372. https://doi.org/10.1074/jbc.271.5.2369
Zhao, Y., and S. L. Young. “Requirement of transforming growth factor-beta (TGF-beta) type II receptor for TGF-beta-induced proliferation and growth inhibition.J Biol Chem 271, no. 5 (February 2, 1996): 2369–72. https://doi.org/10.1074/jbc.271.5.2369.
Zhao, Y., and S. L. Young. “Requirement of transforming growth factor-beta (TGF-beta) type II receptor for TGF-beta-induced proliferation and growth inhibition.J Biol Chem, vol. 271, no. 5, Feb. 1996, pp. 2369–72. Pubmed, doi:10.1074/jbc.271.5.2369.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 2, 1996

Volume

271

Issue

5

Start / End Page

2369 / 2372

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Receptors, Transforming Growth Factor beta
  • Rats
  • Molecular Sequence Data
  • Lung
  • DNA Primers
  • Cells, Cultured
  • Cell Division
  • Biochemistry & Molecular Biology
  • Base Sequence