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Effects of pulse strength and pulse duration on in vitro DNA electromobility.

Publication ,  Journal Article
Zaharoff, DA; Yuan, F
Published in: Bioelectrochemistry (Amsterdam, Netherlands)
April 2004

Interstitial transport of DNA is a rate-limiting step in electric field-mediated gene delivery in vivo. Interstitial transport of macromolecules, such as plasmid DNA, over a distance of several cell layers, is inefficient due to small diffusion coefficient and inadequate convection. Therefore, we explored electric field as a novel driving force for interstitial transport of plasmid DNA. In this study, agarose gels were used to mimic the interstitium in tissues as they had been well characterized and could be prepared reproducibly. We measured the electrophoretic movements of fluorescently labeled plasmid DNA in agarose gels with three different concentrations (1.0%, 2.0% and 3.0%) subjected to electric pulses at three different field strengths (100, 200 and 400 V/cm) and four different pulse durations (10, 50, 75, 99 ms). We observed that: (1) shorter pulses (10 ms) were not as efficient as longer pulses in facilitating plasmid transport through agarose gels; (2) plasmid electromobility reached a plateau at longer pulse durations; and (3) plasmid electromobility increased with applied electric energy, up to a threshold, in all three gels. These data suggested that both pulse strength and duration needed to be adequately high for efficient plasmid transport through extracellular matrix. We also found that electric field was better than concentration gradient of DNA as a driving force for interstitial transport of plasmid DNA.

Duke Scholars

Published In

Bioelectrochemistry (Amsterdam, Netherlands)

DOI

EISSN

1878-562X

ISSN

1567-5394

Publication Date

April 2004

Volume

62

Issue

1

Start / End Page

37 / 45

Related Subject Headings

  • Plasmids
  • Ions
  • In Vitro Techniques
  • Gene Transfer Techniques
  • Gels
  • Fluorescence
  • Electrophoresis, Gel, Pulsed-Field
  • Electrochemistry
  • DNA
  • Analytical Chemistry
 

Citation

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Zaharoff, D. A., & Yuan, F. (2004). Effects of pulse strength and pulse duration on in vitro DNA electromobility. Bioelectrochemistry (Amsterdam, Netherlands), 62(1), 37–45. https://doi.org/10.1016/j.bioelechem.2003.10.011
Zaharoff, David A., and Fan Yuan. “Effects of pulse strength and pulse duration on in vitro DNA electromobility.Bioelectrochemistry (Amsterdam, Netherlands) 62, no. 1 (April 2004): 37–45. https://doi.org/10.1016/j.bioelechem.2003.10.011.
Zaharoff DA, Yuan F. Effects of pulse strength and pulse duration on in vitro DNA electromobility. Bioelectrochemistry (Amsterdam, Netherlands). 2004 Apr;62(1):37–45.
Zaharoff, David A., and Fan Yuan. “Effects of pulse strength and pulse duration on in vitro DNA electromobility.Bioelectrochemistry (Amsterdam, Netherlands), vol. 62, no. 1, Apr. 2004, pp. 37–45. Epmc, doi:10.1016/j.bioelechem.2003.10.011.
Zaharoff DA, Yuan F. Effects of pulse strength and pulse duration on in vitro DNA electromobility. Bioelectrochemistry (Amsterdam, Netherlands). 2004 Apr;62(1):37–45.
Journal cover image

Published In

Bioelectrochemistry (Amsterdam, Netherlands)

DOI

EISSN

1878-562X

ISSN

1567-5394

Publication Date

April 2004

Volume

62

Issue

1

Start / End Page

37 / 45

Related Subject Headings

  • Plasmids
  • Ions
  • In Vitro Techniques
  • Gene Transfer Techniques
  • Gels
  • Fluorescence
  • Electrophoresis, Gel, Pulsed-Field
  • Electrochemistry
  • DNA
  • Analytical Chemistry