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Available space and extracellular transport of macromolecules: effects of pore size and connectedness.

Publication ,  Journal Article
Yuan, F; Krol, A; Tong, S
Published in: Annals of biomedical engineering
December 2001

Molecular exclusion in tumor tissues is one of the limiting factors for drug delivery to tumor cells. It can be quantified by the available volume fraction of solutes (K(AV)). We found in a previous study that K(AV) of dextran in tumor tissues decreased sharply when the molecular weight (MW) of dextran was increased from 40,000 to 70,000. Outside this range, K(AV) was less sensitive to the MW of dextran. To understand the mechanisms of the MW dependence of K(AV), we investigated K(AV) in tissue phantoms composed of tumor cells in 1% agarose gels, and performed numerical simulations of the available volume fraction in pore networks. We found that the MW dependence of K(AV) in tissue phantoms was similar to that in tumor tissues when the volume fraction of cells in the former was approximately 30%. Our numerical simulations revealed that the sharp decrease in K(AV) required two necessary conditions: (i) the existence of at least two populations of pores and (ii) the lack of connectedness of available pores in the interstitial space. Furthermore, results in this study suggest that it is important to consider not only the local structures of pores but also their connectedness in analyses of molecular transport in tissues.

Duke Scholars

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

December 2001

Volume

29

Issue

12

Start / End Page

1150 / 1158

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Porosity
  • Neural Networks, Computer
  • Molecular Weight
  • Models, Biological
  • Mammary Neoplasms, Experimental
  • Macromolecular Substances
  • Humans
  • Extracellular Space
 

Citation

APA
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ICMJE
MLA
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Yuan, F., Krol, A., & Tong, S. (2001). Available space and extracellular transport of macromolecules: effects of pore size and connectedness. Annals of Biomedical Engineering, 29(12), 1150–1158. https://doi.org/10.1114/1.1424915
Yuan, F., A. Krol, and S. Tong. “Available space and extracellular transport of macromolecules: effects of pore size and connectedness.Annals of Biomedical Engineering 29, no. 12 (December 2001): 1150–58. https://doi.org/10.1114/1.1424915.
Yuan F, Krol A, Tong S. Available space and extracellular transport of macromolecules: effects of pore size and connectedness. Annals of biomedical engineering. 2001 Dec;29(12):1150–8.
Yuan, F., et al. “Available space and extracellular transport of macromolecules: effects of pore size and connectedness.Annals of Biomedical Engineering, vol. 29, no. 12, Dec. 2001, pp. 1150–58. Epmc, doi:10.1114/1.1424915.
Yuan F, Krol A, Tong S. Available space and extracellular transport of macromolecules: effects of pore size and connectedness. Annals of biomedical engineering. 2001 Dec;29(12):1150–1158.
Journal cover image

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

December 2001

Volume

29

Issue

12

Start / End Page

1150 / 1158

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Porosity
  • Neural Networks, Computer
  • Molecular Weight
  • Models, Biological
  • Mammary Neoplasms, Experimental
  • Macromolecular Substances
  • Humans
  • Extracellular Space