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Direct in vivo measurement of targeted binding in a human tumor xenograft.

Publication ,  Journal Article
Berk, DA; Yuan, F; Leunig, M; Jain, RK
Published in: Proceedings of the National Academy of Sciences of the United States of America
March 1997

Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue. To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand caused by binding to receptors in vivo. Measurements of mAb affinity to target antigen within human tumor xenografts revealed a saturable binding isotherm, from which an in vivo carcinoembryonic antigen density of 0.56 nmol/g (5.0 x 10(5)/cell) and an association constant of Ka < or = 4 x 10(7) M(-1) were estimated. The present method can be adapted for in vivo studies of cell signaling, targeted drugs, gene therapy, and other processes involving receptor-ligand binding.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

March 1997

Volume

94

Issue

5

Start / End Page

1785 / 1790

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Protein Binding
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Kinetics
  • Immunohistochemistry
  • Humans
  • Fluorescent Dyes
 

Citation

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Berk, D. A., Yuan, F., Leunig, M., & Jain, R. K. (1997). Direct in vivo measurement of targeted binding in a human tumor xenograft. Proceedings of the National Academy of Sciences of the United States of America, 94(5), 1785–1790. https://doi.org/10.1073/pnas.94.5.1785
Berk, D. A., F. Yuan, M. Leunig, and R. K. Jain. “Direct in vivo measurement of targeted binding in a human tumor xenograft.Proceedings of the National Academy of Sciences of the United States of America 94, no. 5 (March 1997): 1785–90. https://doi.org/10.1073/pnas.94.5.1785.
Berk DA, Yuan F, Leunig M, Jain RK. Direct in vivo measurement of targeted binding in a human tumor xenograft. Proceedings of the National Academy of Sciences of the United States of America. 1997 Mar;94(5):1785–90.
Berk, D. A., et al. “Direct in vivo measurement of targeted binding in a human tumor xenograft.Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 5, Mar. 1997, pp. 1785–90. Epmc, doi:10.1073/pnas.94.5.1785.
Berk DA, Yuan F, Leunig M, Jain RK. Direct in vivo measurement of targeted binding in a human tumor xenograft. Proceedings of the National Academy of Sciences of the United States of America. 1997 Mar;94(5):1785–1790.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

March 1997

Volume

94

Issue

5

Start / End Page

1785 / 1790

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Protein Binding
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Kinetics
  • Immunohistochemistry
  • Humans
  • Fluorescent Dyes