Direct in vivo measurement of targeted binding in a human tumor xenograft.

Journal Article (Journal Article)

Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue. To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand caused by binding to receptors in vivo. Measurements of mAb affinity to target antigen within human tumor xenografts revealed a saturable binding isotherm, from which an in vivo carcinoembryonic antigen density of 0.56 nmol/g (5.0 x 10(5)/cell) and an association constant of Ka < or = 4 x 10(7) M(-1) were estimated. The present method can be adapted for in vivo studies of cell signaling, targeted drugs, gene therapy, and other processes involving receptor-ligand binding.

Full Text

Duke Authors

Cited Authors

  • Berk, DA; Yuan, F; Leunig, M; Jain, RK

Published Date

  • March 1997

Published In

Volume / Issue

  • 94 / 5

Start / End Page

  • 1785 - 1790

PubMed ID

  • 9050856

Pubmed Central ID

  • PMC19994

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.5.1785


  • eng