Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin.

Journal Article (Journal Article)

P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin-deficient mice was significantly lower than that in wild-type mice under the basal conditions and post-ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.

Duke Authors

Cited Authors

  • Yamada, S; Mayadas, TN; Yuan, F; Wagner, DD; Hynes, RO; Melder, RJ; Jain, RK

Published Date

  • November 1995

Published In

Volume / Issue

  • 86 / 9

Start / End Page

  • 3487 - 3492

PubMed ID

  • 7579454

Electronic International Standard Serial Number (EISSN)

  • 1528-0020

International Standard Serial Number (ISSN)

  • 0006-4971


  • eng