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Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia.

Publication ,  Journal Article
Hauck, ML; Larsen, RH; Welsh, PC; Zalutsky, MR
Published in: Br J Cancer
March 1998

The high linear energy transfer, alpha-particle-emitting radionuclide astatine-211 (211At) is of interest for certain therapeutic applications; however, because of the 55- to 70-microm path length of its alpha-particles, achieving homogeneous tracer distribution is critical. Hyperthermia may enhance the therapeutic efficacy of alpha-particle endoradiotherapy if it can improve tracer distribution. In this study, we have investigated whether hyperthermia increased the cytotoxicity of an 211At-labelled monoclonal antibody (MAb) in tumour spheroids with a radius (approximately 100 microm) greater than the range of 211At alpha-particles. Hyperthermia for 1 h at 42 degrees C was used because this treatment itself resulted in no regrowth delay. Radiolabelled chimeric MAb 81C6 reactive with the extracellular matrix antigen tenascin was added to spheroids grown from the D-247 MG human glioma cell line at activity concentrations ranging from 0.125 to 250 kBq ml(-1). A significant regrowth delay was observed at 125 and 250 kBq ml(-1) in both hyperthermia-treated and untreated spheroids. For groups receiving hyperthermia, no increase in cytotoxicity was seen compared with normothermic controls at any activity concentration. These results and those from autoradiographs indicate that hyperthermia at 42 degrees C for 1 h had no significant effect on the uptake or distribution of this antitenascin MAb in D-247 MG spheroids.

Duke Scholars

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

March 1998

Volume

77

Issue

5

Start / End Page

753 / 759

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tenascin
  • Recombinant Fusion Proteins
  • Radioimmunotherapy
  • Organoids
  • Oncology & Carcinogenesis
  • Mice
  • Immunoglobulin G
  • Hyperthermia, Induced
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Hauck, M. L., Larsen, R. H., Welsh, P. C., & Zalutsky, M. R. (1998). Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia. Br J Cancer, 77(5), 753–759. https://doi.org/10.1038/bjc.1998.123
Hauck, M. L., R. H. Larsen, P. C. Welsh, and M. R. Zalutsky. “Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia.Br J Cancer 77, no. 5 (March 1998): 753–59. https://doi.org/10.1038/bjc.1998.123.
Hauck ML, Larsen RH, Welsh PC, Zalutsky MR. Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia. Br J Cancer. 1998 Mar;77(5):753–9.
Hauck, M. L., et al. “Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia.Br J Cancer, vol. 77, no. 5, Mar. 1998, pp. 753–59. Pubmed, doi:10.1038/bjc.1998.123.
Hauck ML, Larsen RH, Welsh PC, Zalutsky MR. Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia. Br J Cancer. 1998 Mar;77(5):753–759.

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

March 1998

Volume

77

Issue

5

Start / End Page

753 / 759

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tenascin
  • Recombinant Fusion Proteins
  • Radioimmunotherapy
  • Organoids
  • Oncology & Carcinogenesis
  • Mice
  • Immunoglobulin G
  • Hyperthermia, Induced
  • Humans