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Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.

Publication ,  Journal Article
Kenan, DJ; Walsh, EB; Meyers, SR; O'Toole, GA; Carruthers, EG; Lee, WK; Zauscher, S; Prata, CAH; Grinstaff, MW
Published in: Chemistry & biology
July 2006
Published version (DOI)

Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.

Duke Scholars

Stefan Zauscher
Author Stefan Zauscher Thomas Lord Department of Mechanical Engineering and Materia ...
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Published In

Chemistry & biology

DOI

10.1016/j.chembiol.2006.06.013

EISSN

1879-1301

ISSN

1074-5521

Publication Date

July 2006

Volume

13

Issue

7

Start / End Page

695 / 700

Related Subject Headings

  • Staphylococcus aureus
  • Polyethylene Glycols
  • Peptides
  • Organic Chemistry
  • Molecular Sequence Data
  • Microscopy, Atomic Force
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Bacterial Adhesion
  • Amino Acid Sequence
 

Citation

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MLA
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Kenan, D. J., Walsh, E. B., Meyers, S. R., O’Toole, G. A., Carruthers, E. G., Lee, W. K., … Grinstaff, M. W. (2006). Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells. Chemistry & Biology, 13(7), 695–700. https://doi.org/10.1016/j.chembiol.2006.06.013
Kenan, Daniel J., Elisabeth B. Walsh, Steven R. Meyers, George A. O’Toole, Erin G. Carruthers, Woo K. Lee, Stefan Zauscher, Carla A. H. Prata, and Mark W. Grinstaff. “Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.” Chemistry & Biology 13, no. 7 (July 2006): 695–700. https://doi.org/10.1016/j.chembiol.2006.06.013.
Kenan DJ, Walsh EB, Meyers SR, O’Toole GA, Carruthers EG, Lee WK, et al. Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells. Chemistry & biology. 2006 Jul;13(7):695–700.
Kenan, Daniel J., et al. “Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.” Chemistry & Biology, vol. 13, no. 7, July 2006, pp. 695–700. Epmc, doi:10.1016/j.chembiol.2006.06.013.
Kenan DJ, Walsh EB, Meyers SR, O’Toole GA, Carruthers EG, Lee WK, Zauscher S, Prata CAH, Grinstaff MW. Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells. Chemistry & biology. 2006 Jul;13(7):695–700.

Published In

Chemistry & biology

DOI

10.1016/j.chembiol.2006.06.013

EISSN

1879-1301

ISSN

1074-5521

Publication Date

July 2006

Volume

13

Issue

7

Start / End Page

695 / 700

Related Subject Headings

  • Staphylococcus aureus
  • Polyethylene Glycols
  • Peptides
  • Organic Chemistry
  • Molecular Sequence Data
  • Microscopy, Atomic Force
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Bacterial Adhesion
  • Amino Acid Sequence