Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells.

Journal Article (Journal Article)

Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.

Full Text

Duke Authors

Cited Authors

  • Kenan, DJ; Walsh, EB; Meyers, SR; O'Toole, GA; Carruthers, EG; Lee, WK; Zauscher, S; Prata, CAH; Grinstaff, MW

Published Date

  • July 2006

Published In

Volume / Issue

  • 13 / 7

Start / End Page

  • 695 - 700

PubMed ID

  • 16873017

Pubmed Central ID

  • 16873017

Electronic International Standard Serial Number (EISSN)

  • 1879-1301

International Standard Serial Number (ISSN)

  • 1074-5521

Digital Object Identifier (DOI)

  • 10.1016/j.chembiol.2006.06.013


  • eng