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Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney.

Publication ,  Journal Article
Xing, Y; Pua, EC; Lu, X; Zhong, P
Published in: Biochemical and biophysical research communications
August 2009

The synergistic combination of hydrodynamic-based gene delivery and ultrasound was investigated to achieve improved gene transfer to the kidney. Plasmids encoding firefly luciferase and Erythropoietin (EPO) gene were delivered into the left kidney of rats by single or combinative application of renal vein hydrodynamic injection and ultrasound treatment with or without the addition of ultrasound contrast agents (UCA). Ultrasound exposure was found to enhance the efficiency of hydrodynamic-based gene delivery for both luciferase and EPO expression. An ultrasound exposure intensity of 2 W/cm2 at 10% duty cycle for 15 min, produced a maximal gene expression 4.5 times higher than hydrodynamic delivery alone. Duration, location, and tissue-specificity of gene expression were not changed by ultrasound exposure. Application of UCA reduced the intensity and exposure duration of ultrasound treatment needed for optimal expression. Appropriate application of ultrasound and UCA did not alter histological structure or impair physiological function of the treated kidney.

Duke Scholars

Published In

Biochemical and biophysical research communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

August 2009

Volume

386

Issue

1

Start / End Page

217 / 222

Related Subject Headings

  • Ultrasonics
  • Rats, Wistar
  • Rats
  • Plasmids
  • Male
  • Luciferases
  • Kidney
  • Injections, Intravenous
  • Genetic Vectors
  • Genetic Therapy
 

Citation

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Xing, Y., Pua, E. C., Lu, X., & Zhong, P. (2009). Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. Biochemical and Biophysical Research Communications, 386(1), 217–222. https://doi.org/10.1016/j.bbrc.2009.06.020
Xing, Yifei, Eric C. Pua, Xiaochun Lu, and Pei Zhong. “Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney.Biochemical and Biophysical Research Communications 386, no. 1 (August 2009): 217–22. https://doi.org/10.1016/j.bbrc.2009.06.020.
Xing Y, Pua EC, Lu X, Zhong P. Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. Biochemical and biophysical research communications. 2009 Aug;386(1):217–22.
Xing, Yifei, et al. “Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney.Biochemical and Biophysical Research Communications, vol. 386, no. 1, Aug. 2009, pp. 217–22. Epmc, doi:10.1016/j.bbrc.2009.06.020.
Xing Y, Pua EC, Lu X, Zhong P. Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. Biochemical and biophysical research communications. 2009 Aug;386(1):217–222.
Journal cover image

Published In

Biochemical and biophysical research communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

August 2009

Volume

386

Issue

1

Start / End Page

217 / 222

Related Subject Headings

  • Ultrasonics
  • Rats, Wistar
  • Rats
  • Plasmids
  • Male
  • Luciferases
  • Kidney
  • Injections, Intravenous
  • Genetic Vectors
  • Genetic Therapy