The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.

Published

Journal Article

We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF-like motifs. We assayed two independent mutant alleles--'Jackson reeler', which has a deletion of the entire gene, and 'Orleans reeler' which exhibits a 220 bp deletion in the open reading frame, including the second EGF-like motif and resulting in a frame shift. In situ hybridization reveals that the transcript is detected exclusively in the pioneer neurons which guide neuronal cell migration along the radial array. Our findings offer an explanation for how the reeler mutant phenotype causes a disturbance of the complex architecture of the neuronal network.

Full Text

Cited Authors

  • Hirotsune, S; Takahara, T; Sasaki, N; Hirose, K; Yoshiki, A; Ohashi, T; Kusakabe, M; Murakami, Y; Muramatsu, M; Watanabe, S

Published Date

  • May 1995

Published In

Volume / Issue

  • 10 / 1

Start / End Page

  • 77 - 83

PubMed ID

  • 7647795

Pubmed Central ID

  • 7647795

Electronic International Standard Serial Number (EISSN)

  • 1546-1718

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng0595-77

Language

  • eng