Faithful expression of GFP from the PLCbeta2 promoter in a functional class of taste receptor cells.

Published

Journal Article

Phospholipase C-type beta2 (PLCbeta2) is expressed in a subset of cells within mammalian taste buds. This enzyme is involved in the transduction of sweet, bitter, and umami stimuli and thus is believed to be a marker for gustatory sensory receptor cells. We have developed transgenic mice expressing green fluorescent protein (GFP) under the control of the PLCbeta2 promoter to enable one to identify these cells and record their physiological activity in living preparations. Expression of GFP (especially in lines with more than one copy integrated) is strong enough to be detected in intact tissue preparations using epifluorescence microscopy. By immunohistochemistry, we confirmed that the overwhelming majority of cells expressing GFP are those that endogenously express PLCbeta2. Expression of the GFP transgene in circumvallate papillae occurs at about the same time during development as endogenous PLCbeta2 expression. When loaded with a calcium-sensitive dye in situ, GFP-positive taste cells produce typical Ca2+ responses to a taste stimulus, the bitter compound cycloheximide. These PLCbeta2 promoter-GFP transgenic lines promise to be useful for studying taste transduction, sensory signal processing, and taste bud development.

Full Text

Cited Authors

  • Kim, JW; Roberts, C; Maruyama, Y; Berg, S; Roper, S; Chaudhari, N

Published Date

  • March 2006

Published In

Volume / Issue

  • 31 / 3

Start / End Page

  • 213 - 219

PubMed ID

  • 16394244

Pubmed Central ID

  • 16394244

Electronic International Standard Serial Number (EISSN)

  • 1464-3553

International Standard Serial Number (ISSN)

  • 0379-864X

Digital Object Identifier (DOI)

  • 10.1093/chemse/bjj021

Language

  • eng