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Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells.

Publication ,  Journal Article
Kiefer, T; Ram, PT; Yuan, L; Hill, SM
Published in: Breast Cancer Res Treat
January 2002

We have previously demonstrated that the pineal hormone, melatonin, can inhibit the growth of estrogen receptor-alpha (ERalpha)-positive breast cancer cells and suppress ERalpha gene transcription. To investigate the relationship between the estrogen response pathway and melatonin's growth inhibition, ERalpha-positive MCF-7 human breast cancer cells were transiently transfected with an estrogen response element (ERE) luciferase reporter construct and then treated with melatonin (10(-9)-10(-6) M) for 30 min followed by 10(-9) M 17-beta-estradiol (E2) or treated with each compound alone. Melatonin pre-treatment significantly reduced E2-induced ERalpha transactivation and ERalpha-ERE binding activity. We also conducted experiments to determine if melatonin modulates cAMP levels in MCF-7 cells. Melatonin inhibited the forskolin-induced and E2-induced elevation of cAMP levels by 57 and 45%, respectively. These data indicate that melatonin can act as a biological modifier to affect ERalpha transcriptional activity by regulating signal transduction pathways which impinge on the ERalpha and by altering E2-mediated ERalpha transactivation and ERalpha DNA binding activity.

Duke Scholars

Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

January 2002

Volume

71

Issue

1

Start / End Page

37 / 45

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Signal Transduction
  • Receptors, Estrogen
  • Oncology & Carcinogenesis
  • Melatonin
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Female
  • Estrogen Receptor alpha
 

Citation

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Kiefer, T., Ram, P. T., Yuan, L., & Hill, S. M. (2002). Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells. Breast Cancer Res Treat, 71(1), 37–45. https://doi.org/10.1023/a:1013301408464
Kiefer, T., P. T. Ram, L. Yuan, and S. M. Hill. “Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells.Breast Cancer Res Treat 71, no. 1 (January 2002): 37–45. https://doi.org/10.1023/a:1013301408464.
Kiefer T, Ram PT, Yuan L, Hill SM. Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells. Breast Cancer Res Treat. 2002 Jan;71(1):37–45.
Kiefer, T., et al. “Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells.Breast Cancer Res Treat, vol. 71, no. 1, Jan. 2002, pp. 37–45. Pubmed, doi:10.1023/a:1013301408464.
Kiefer T, Ram PT, Yuan L, Hill SM. Melatonin inhibits estrogen receptor transactivation and cAMP levels in breast cancer cells. Breast Cancer Res Treat. 2002 Jan;71(1):37–45.
Journal cover image

Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

January 2002

Volume

71

Issue

1

Start / End Page

37 / 45

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Signal Transduction
  • Receptors, Estrogen
  • Oncology & Carcinogenesis
  • Melatonin
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Female
  • Estrogen Receptor alpha