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Identification of an enhancer element in the estrogen receptor upstream region: implications for regulation of ER transcription in breast cancer.

Publication ,  Journal Article
Cohn, CS; Sullivan, JA; Kiefer, T; Hill, SM
Published in: Mol Cell Endocrinol
December 20, 1999

The estrogen receptor (ER) serves as a diagnostic marker for the treatment of breast cancer. Patients with ER-positive breast tumors are likely to respond to hormonal therapies, while ER-negative breast cancers are resistant to endocrine therapies. Most ER-negative tumors do not express detectable levels of ER transcript, highlighting the importance of transcriptional regulation. A novel regulatory element which resembles a steroid hormone response element has been identified in the 5'-flanking region of the human ER gene. We observed 3- to 5-fold higher specific binding to this element in nuclear extracts from ER-expressing MCF-7 breast cancer cells compared to ER-negative MDA-MB-231 breast tumor cells. We termed the factor(s) which bind to this cis-element estrogen receptor upstream binding factor-1 (ERUBF-1). In transient transfection assays in MCF-7 cells, the ERUBF-1 binding site elicited a 20-fold increase in luciferase activity over the ER P1, promoter. This enhancer element was significantly more active in the ER-positive MCF-7 cell line compared to the ER-negative MDA-MB-231 cell line. These data indicate that ERUBF-1 plays an important role in the transcriptional regulation of the ER gene in breast cancer.

Duke Scholars

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

December 20, 1999

Volume

158

Issue

1-2

Start / End Page

25 / 36

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Receptors, Estrogen
  • Protein Binding
  • Neoplasms, Hormone-Dependent
  • Molecular Sequence Data
  • Luciferases
  • Humans
  • Genes, Reporter
  • Female
 

Citation

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Cohn, C. S., Sullivan, J. A., Kiefer, T., & Hill, S. M. (1999). Identification of an enhancer element in the estrogen receptor upstream region: implications for regulation of ER transcription in breast cancer. Mol Cell Endocrinol, 158(1–2), 25–36. https://doi.org/10.1016/s0303-7207(99)00187-2
Cohn, C. S., J. A. Sullivan, T. Kiefer, and S. M. Hill. “Identification of an enhancer element in the estrogen receptor upstream region: implications for regulation of ER transcription in breast cancer.Mol Cell Endocrinol 158, no. 1–2 (December 20, 1999): 25–36. https://doi.org/10.1016/s0303-7207(99)00187-2.
Cohn, C. S., et al. “Identification of an enhancer element in the estrogen receptor upstream region: implications for regulation of ER transcription in breast cancer.Mol Cell Endocrinol, vol. 158, no. 1–2, Dec. 1999, pp. 25–36. Pubmed, doi:10.1016/s0303-7207(99)00187-2.
Journal cover image

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

December 20, 1999

Volume

158

Issue

1-2

Start / End Page

25 / 36

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Receptors, Estrogen
  • Protein Binding
  • Neoplasms, Hormone-Dependent
  • Molecular Sequence Data
  • Luciferases
  • Humans
  • Genes, Reporter
  • Female