Near-infrared spectrometry of abdominal aortic aneurysm in the ApoE-/- mouse.

Published

Journal Article

Abdominal aortic aneurysms (AAAs) occur in 5-7% of people over age 60 in the United States. Early intervention in the disease process could have a significant impact on the incidence of complications and on patient survival, but identifying incipient aneurysms can be difficult. ApoE knockout mice develop AAAs following infusion of angiotensin II (AngII) by osmotic minipump into the subcutaneous space of mice at doses ranging from 500 to 1000 ng kg(-1) min(-1) for 7-28 days. These mice are used as models of AAA development. This study tested the hypothesis that near-IR spectrometry and PCR can determine AngII dose (SEE = 26 ng kg(-1) min(-1), SEP = 37 ng kg(-1) min(-1), r2 = 0.99) and collagen/elastin (C/E) ratio (SEE = 0.38, SEP = 0.39, r2 = 0.85) in mouse aortas.

Full Text

Duke Authors

Cited Authors

  • Urbas, A; Manning, MW; Daugherty, A; Cassis, LA; Lodder, RA

Published Date

  • July 15, 2003

Published In

Volume / Issue

  • 75 / 14

Start / End Page

  • 3650 - 3655

PubMed ID

  • 14570222

Pubmed Central ID

  • 14570222

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac034113n

Language

  • eng

Conference Location

  • United States