A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein.

Published

Journal Article

The sigma initiation factor sigma70 of Escherichia coli acts not only in promoter recognition and DNA strand opening, but also to mediate the transformation of RNA polymerase (RNAP) to an antiterminating form by the phage lambda gene Q protein. Q is able to bind and modify RNAP when alpha70, still present in the initially elongating enzyme, recognizes a repeat of the -10 promoter element and induces a transcription pause. We have isolated mutations in the rpoD gene for sigma70 that impair Q function because they reduce the ability of sigma70 to recognize the downstream pause site. These mutations identify a locus of sigma70 that is important for the formation and stability of open promoter complex, likely because it mediates protein interactions with RNAP core.

Full Text

Duke Authors

Cited Authors

  • Ko, DC; Marr, MT; Guo, J; Roberts, JW

Published Date

  • October 1998

Published In

Volume / Issue

  • 12 / 20

Start / End Page

  • 3276 - 3285

PubMed ID

  • 9784501

Pubmed Central ID

  • 9784501

Electronic International Standard Serial Number (EISSN)

  • 1549-5477

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.12.20.3276

Language

  • eng