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A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein.

Publication ,  Journal Article
Ko, DC; Marr, MT; Guo, J; Roberts, JW
Published in: Genes Dev
October 15, 1998

The sigma initiation factor sigma70 of Escherichia coli acts not only in promoter recognition and DNA strand opening, but also to mediate the transformation of RNA polymerase (RNAP) to an antiterminating form by the phage lambda gene Q protein. Q is able to bind and modify RNAP when alpha70, still present in the initially elongating enzyme, recognizes a repeat of the -10 promoter element and induces a transcription pause. We have isolated mutations in the rpoD gene for sigma70 that impair Q function because they reduce the ability of sigma70 to recognize the downstream pause site. These mutations identify a locus of sigma70 that is important for the formation and stability of open promoter complex, likely because it mediates protein interactions with RNAP core.

Duke Scholars

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

October 15, 1998

Volume

12

Issue

20

Start / End Page

3276 / 3285

Location

United States

Related Subject Headings

  • Viral Proteins
  • Transcription, Genetic
  • Sigma Factor
  • Protein Binding
  • Promoter Regions, Genetic
  • Phenylalanine
  • Peptide Chain Termination, Translational
  • Mutagenesis
  • Molecular Sequence Data
  • Models, Molecular
 

Citation

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Ko, D. C., Marr, M. T., Guo, J., & Roberts, J. W. (1998). A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein. Genes Dev, 12(20), 3276–3285. https://doi.org/10.1101/gad.12.20.3276
Ko, D. C., M. T. Marr, J. Guo, and J. W. Roberts. “A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein.Genes Dev 12, no. 20 (October 15, 1998): 3276–85. https://doi.org/10.1101/gad.12.20.3276.
Ko, D. C., et al. “A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein.Genes Dev, vol. 12, no. 20, Oct. 1998, pp. 3276–85. Pubmed, doi:10.1101/gad.12.20.3276.
Ko DC, Marr MT, Guo J, Roberts JW. A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein. Genes Dev. 1998 Oct 15;12(20):3276–3285.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

October 15, 1998

Volume

12

Issue

20

Start / End Page

3276 / 3285

Location

United States

Related Subject Headings

  • Viral Proteins
  • Transcription, Genetic
  • Sigma Factor
  • Protein Binding
  • Promoter Regions, Genetic
  • Phenylalanine
  • Peptide Chain Termination, Translational
  • Mutagenesis
  • Molecular Sequence Data
  • Models, Molecular