Human embryos derived from in vitro and in vivo matured oocytes: analysis for chromosomal abnormalities and nuclear morphology.

Journal Article (Journal Article)

PURPOSE: To determine whether embryos resulting from oocytes matured in vitro have a higher incidence of nuclear and/or genetic abnormalities compared to embryos resulting from oocytes matured in vivo. METHODS: Fluorescence in situ hybridization analysis for chromosomes X, Y, and 18 was used to compare the rates of aneuploidy, mosaicism, and nuclear abnormalities in embryos derived from oocytes that were prophase I at aspiration (immature group) to that observed in embryos resulting from oocytes that were metaphase I or II at aspiration (mature group). RESULTS: Based on nuclear morphology, significantly more embryos in the mature group (23%) were classified as normal compared to embryos in the immature group (3%). No difference was found in the rate of aneuploidy or in the incidence of mosaicism involving these chromosomes. CONCLUSIONS: These findings suggest that few embryos derived from prophase I oocytes collected following ovarian stimulation are morphologically normal.

Full Text

Duke Authors

Cited Authors

  • DeScisciolo, C; Wright, DL; Mayer, JF; Gibbons, W; Muasher, SJ; Lanzendorf, SE

Published Date

  • May 2000

Published In

Volume / Issue

  • 17 / 5

Start / End Page

  • 284 - 292

PubMed ID

  • 10976416

Pubmed Central ID

  • PMC3455201

International Standard Serial Number (ISSN)

  • 1058-0468

Digital Object Identifier (DOI)

  • 10.1023/a:1009414517546


  • eng

Conference Location

  • Netherlands