Is the timing of implantation affected by zona pellucida micromanipulation?

Journal Article (Journal Article)

PURPOSE: Our purpose was to examine the timing of implantation and early embryo development following uterine transfer of oocytes/embryos previously subjected to zona pellucida micromanipulation. METHODS: A total of 68 singleton pregnancies resulting from IVF and embryo transfer with/without micromanipulation. Patients were divided into four groups according to the type of micromanipulation technique: assisted hatching, embryo biopsy, intracytoplasmic sperm injection, and no micromanipulation (control group). Serial serum beta-hCG levels were measured between 10 and 25 days after fertilization and log-transformed. Linear regression analyses were performed and extrapolated to hCG = 10 mIU/ml (hCG10) to estimate detectable implantation. The slopes of the regression lines were used to estimate the rising speed of hCG, an indirect sign of embryo development. RESULTS: There were no significant differences among groups with respect to hCG10, the slopes or intercepts of the regression lines. CONCLUSIONS: Various oocyte/embryo microsurgical procedures used in ART involving zona pellucida manipulation do not appear to affect the timing of implantation or early embryo development.

Full Text

Duke Authors

Cited Authors

  • Hsu, MI; Barroso, G; Mayer, J; Lanzendorf, S; Gibbons, WE; Muasher, S; Oehninger, S

Published Date

  • January 2000

Published In

Volume / Issue

  • 17 / 1

Start / End Page

  • 34 - 38

PubMed ID

  • 10754781

Pubmed Central ID

  • PMC3455187

International Standard Serial Number (ISSN)

  • 1058-0468

Digital Object Identifier (DOI)

  • 10.1023/a:1009497929994


  • eng

Conference Location

  • Netherlands