Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes.

Published

Journal Article

OBJECTIVE: To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development. DESIGN: Prospective evaluation of the procedure in consenting IVF patients. SETTING: Assisted reproductive technology program. PATIENT(S): The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was > or = 40 years. INTERVENTION(S): Transfer of donor egg cytoplasm from frozen-thawed oocytes into the oocytes of infertile recipients. MAIN OUTCOME MEASURE(S): Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome. RESULT(S): Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality. CONCLUSION(S): Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.

Full Text

Duke Authors

Cited Authors

  • Lanzendorf, SE; Mayer, JF; Toner, J; Oehninger, S; Saffan, DS; Muasher, S

Published Date

  • March 1999

Published In

Volume / Issue

  • 71 / 3

Start / End Page

  • 575 - 577

PubMed ID

  • 10065803

Pubmed Central ID

  • 10065803

Electronic International Standard Serial Number (EISSN)

  • 1556-5653

International Standard Serial Number (ISSN)

  • 0015-0282

Digital Object Identifier (DOI)

  • 10.1016/s0015-0282(98)00504-4

Language

  • eng