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Fertilization and in vitro development of cryopreserved human prophase I oocytes.

Publication ,  Journal Article
Toth, TL; Baka, SG; Veeck, LL; Jones, HW; Muasher, S; Lanzendorf, SE
Published in: Fertil Steril
May 1994

OBJECTIVE: To determine the potential for in vitro maturation, fertilization, and cleavage after cryopreservation of immature, prophase I human oocytes. DESIGN: Immature oocytes obtained in excess of the number required by the patient were randomized and cryopreserved at the prophase I stage or cultured as control. After thawing and maturation in vitro, test and control oocytes were inseminated with husband's sperm and evaluated for fertilization and cleavage in vitro. SETTING: In vitro fertilization program. PATIENTS: Consenting patients undergoing controlled ovarian hyperstimulation for the purposes of IVF. MAIN OUTCOME MEASURES: Rates of maturation to metaphase II, fertilization, and cleavage were compared between control and cryopreserved oocytes. RESULTS: Upon thaw, 58.5% (72/123) of prophase I oocytes were viable. Control oocytes demonstrated a 74.8% (98/131) maturation rate to metaphase II, a 56.5% (52/92) fertilization rate, and an 11.5% (6/52) blastocyst rate. Cryopreserved oocytes showed a 83.3% (60/72) rate of maturation, a 57.7% (30/52) fertilization rate, and a 3.3% (1/30) blastocyst rate. No significant differences were noted between any of these parameters. CONCLUSIONS: These results demonstrate that prophase I oocytes from stimulated IVF cycles are able to survive cryopreservation and resume meiosis to achieve full nuclear maturation post-thaw. In addition, cryopreserved oocytes retain the same capacity for fertilization and development as control oocytes.

Duke Scholars

Published In

Fertil Steril

DOI

ISSN

0015-0282

Publication Date

May 1994

Volume

61

Issue

5

Start / End Page

891 / 894

Location

United States

Related Subject Headings

  • Tissue Preservation
  • Prophase
  • Ovulation Induction
  • Ovary
  • Oocytes
  • Obstetrics & Reproductive Medicine
  • Metaphase
  • In Vitro Techniques
  • Humans
  • Fertilization in Vitro
 

Citation

APA
Chicago
ICMJE
MLA
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Toth, T. L., Baka, S. G., Veeck, L. L., Jones, H. W., Muasher, S., & Lanzendorf, S. E. (1994). Fertilization and in vitro development of cryopreserved human prophase I oocytes. Fertil Steril, 61(5), 891–894. https://doi.org/10.1016/s0015-0282(16)56702-8
Toth, T. L., S. G. Baka, L. L. Veeck, H. W. Jones, S. Muasher, and S. E. Lanzendorf. “Fertilization and in vitro development of cryopreserved human prophase I oocytes.Fertil Steril 61, no. 5 (May 1994): 891–94. https://doi.org/10.1016/s0015-0282(16)56702-8.
Toth TL, Baka SG, Veeck LL, Jones HW, Muasher S, Lanzendorf SE. Fertilization and in vitro development of cryopreserved human prophase I oocytes. Fertil Steril. 1994 May;61(5):891–4.
Toth, T. L., et al. “Fertilization and in vitro development of cryopreserved human prophase I oocytes.Fertil Steril, vol. 61, no. 5, May 1994, pp. 891–94. Pubmed, doi:10.1016/s0015-0282(16)56702-8.
Toth TL, Baka SG, Veeck LL, Jones HW, Muasher S, Lanzendorf SE. Fertilization and in vitro development of cryopreserved human prophase I oocytes. Fertil Steril. 1994 May;61(5):891–894.
Journal cover image

Published In

Fertil Steril

DOI

ISSN

0015-0282

Publication Date

May 1994

Volume

61

Issue

5

Start / End Page

891 / 894

Location

United States

Related Subject Headings

  • Tissue Preservation
  • Prophase
  • Ovulation Induction
  • Ovary
  • Oocytes
  • Obstetrics & Reproductive Medicine
  • Metaphase
  • In Vitro Techniques
  • Humans
  • Fertilization in Vitro