The prevalence of human immunodeficiency virus in patients and their spouses entering a large in vitro fertilization program.

Journal Article (Journal Article)

Between September 1987 and August 1989, all patients and their spouses entering our in vitro fertilization (IVF) program were screened for the human immunodeficiency virus (HIV) using the enzyme-linked immunosorbent assay (ELISA). Of 848 patients and 848 spouses tested, all but 4 patients and 1 husband tested negative. Of those who tested positive on repeat testing with ELISA, only one was positive on Western blotting (HIV prevalence, 0.59 per 1000). During this same time period 1187 samples of human cord blood were used to make tissue culture medium for the IVF embryology laboratory. One sample was discarded because of positive HIV on ELISA and Western blotting; two other samples were discarded because of positivity to the hepatitis B surface antigen. While we believe that routine HIV screening of IVF patients and their spouses is indicated, this population is of low risk for HIV positivity. Furthermore, continued screening of human sera used to make tissue culture media for IVF is mandatory.

Full Text

Duke Authors

Cited Authors

  • Edelstein, MC; Kruithoff, C; Brothman, L; Veeck, LL; Alexander, NJ; Muasher, SJ

Published Date

  • October 1990

Published In

Volume / Issue

  • 7 / 5

Start / End Page

  • 254 - 256

PubMed ID

  • 2254688

International Standard Serial Number (ISSN)

  • 0740-7769

Digital Object Identifier (DOI)

  • 10.1007/BF01129529


  • eng

Conference Location

  • United States