Rapid heterotrophic ossification with cryopreserved poly(ethylene glycol-) microencapsulated BMP2-expressing MSCs


Journal Article

Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs. © 2012 Jennifer Mumaw et al.

Full Text

Duke Authors

Cited Authors

  • Mumaw, J; Jordan, ET; Sonnet, C; Olabisi, RM; Olmsted-Davis, EA; Davis, AR; Peroni, JF; West, JL; West, F; Lu, Y; Stice, SL

Published Date

  • March 21, 2012

Published In

Electronic International Standard Serial Number (EISSN)

  • 1687-8795

International Standard Serial Number (ISSN)

  • 1687-8787

Digital Object Identifier (DOI)

  • 10.1155/2012/861794

Citation Source

  • Scopus