Protease-activated quantum dot probes


Journal Article

We demonstrate a novel quantum dot based probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This probe may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically-degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Peptide cleavage results in release of AuNPs and restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 hours of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

Full Text

Duke Authors

Cited Authors

  • Chang, E; Sun, J; Miller, JS; Yu, WW; Calvin, VL; West, JL; Drezek, R

Published Date

  • June 22, 2006

Published In

Volume / Issue

  • 6191 /

International Standard Serial Number (ISSN)

  • 1605-7422

Digital Object Identifier (DOI)

  • 10.1117/12.660485

Citation Source

  • Scopus