Nitric oxide-generating poly(ethylene glycol) copolymers for prevention of restenosis
The ability of nitric oxide (NO)-generating hydrogels to inhibit intimal thickening, a major component of restenosis, in a rat carotid injury model, was analyzed. NO-releasing materials were incubated at 37°C in HEPES buffer and assayed for NO release using the Griess assay. To examine cell proliferation, histological sections were immunostained for proliferating cell nuclear antigen (PCNA). Vessel re-endothelialization appeared to be slightly enhanced in the presence of the NO-generating hydrodgels. The results show that the combined effects of NO-generating hydrogels make them well suited for use as a stent coating or in endoluminal paving to prevent restenosis.