Nitric oxide-generating poly(ethylene glycol) copolymers for prevention of restenosis
The use of nitric oxide-generating poly(ethylene glycol) (PEG) copolymers for prevention of restenosis was analyzed. PEG-NO-nucleophile was formed by reacting PEG N-hydroxysuccinimide monoacrylate (ACRL-PEG-NHS; MW 3.4 kDa) with cysteine (Cys). The resultant copolymer was dissolved in water and reacted with an equimolar amount of sodium nitrate at pH 2 to form the hydrogel precursor, PEG-Cys-NO. The results show that the PEG-Cys-NO hydrogels produced nitric oxide over a period of approximately 2 hr at pH 7.4, with most of the release occurring within the first 30 min.