VEGF-transfected vascular smooth muscle cells promote endothelialization and capillary formation
The genetic modification of vascular smooth muscle cells (VSMC) to produce vascular endothelial growth factor (VEGF) was analyzed. VSMCs were isolated from the thoracic aorate of adult sprague-dawley rats and transfected with a retrovirus that encodes VEGF or green fluorescent protein (GFP). VSMCs were seeded into 6-well at 25×10 3 cells/cm 2 and allowed to adhere overnight. The results show that seeding of VEGF-transfected SMCs onto the luminal surface of small-diameter TEVGs may promote spontaneous endothelializarion thereby improving the patency rate of the graft.