A collagen-based gene delivery system with polyethylenimine-DNA complexes
A gene delivery system was developed by embedding polyethyleimine-DNA (PEI-DNA) complexes into cell-seeded collagen gels. The main objective of the development was to enhance endothelialization of tissue engineered vascular grafts (TEVG). PEI solution was added dropwise to DNA at a molar ratio of 7.5:1. A prolonged delivery period of one month was achieved by our gene delivery system. The amount of vascular endothelial growth factor (VEGF) obtained was effective on stimulating profliferation of endothelial cells (EC).