A whole blood immunoassay using gold nanoshells.


Journal Article

A rapid immunoassay capable of detecting analyte within complex biological media without any sample preparation is described. This was accomplished using gold nanoshells, layered dielectric-metal nanoparticles whose optical resonance is a function of the relative size of its constituent layers. Aggregation of antibody/nanoshell conjugates with extinction spectra in the near-infrared was monitored spectroscopically in the presence of analyte. Successful detection of immunoglobulins was achieved in saline, serum, and whole blood. This system constitutes a simple immunoassay capable of detecting sub-nanogram-per-milliliter quantities of various analytes in different media within 10-30 min.

Full Text

Duke Authors

Cited Authors

  • Hirsch, LR; Jackson, JB; Lee, A; Halas, NJ; West, JL

Published Date

  • May 2003

Published In

Volume / Issue

  • 75 / 10

Start / End Page

  • 2377 - 2381

PubMed ID

  • 12918980

Pubmed Central ID

  • 12918980

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac0262210


  • eng