A Rapid, Whole Blood Immunoassay using Metal Nanoshells


Journal Article

Using metal nanoshells as an immunoassay substrate, we describe an immunoassay capable of detecting blood borne analyte on the order of minutes. Near infrared resonant gold nanoshells were labeled with antibodies specific to rabbit IgG analyte. The antibody-nanoshell conjugates were detectable via near infrared photometry in solution with saline, serum, and whole blood. Addition of analyte induced aggregation of antibody-nanoshell conjugates, causing a decrease in the original nanoshell near infrared resonance. Aggregation proceeded in a concentration dependent fasion in all three mediums (saline, serum, whole blood), permitting quantitative detection of analyte within 10 - 30 minutes and sensitivities < 1 ng/mL.

Duke Authors

Cited Authors

  • Hirsch, LR; West, JL; Jackson, JB; Lee, A; Halas, NJ

Published Date

  • December 1, 2003

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 3442 - 3443

International Standard Serial Number (ISSN)

  • 0589-1019

Citation Source

  • Scopus