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Isolation and generation of human dendritic cells.

Publication ,  Journal Article
Nair, S; Archer, GE; Tedder, TF
Published in: Curr Protoc Immunol
November 2012

Dendritic cells are highly specialized antigen-presenting cells (APC), which may be isolated or generated from human blood mononuclear cells. Although mature blood dendritic cells normally represent ∼0.2% of human blood mononuclear cells, their frequency can be greatly increased using the cell enrichment methods described in this unit. More highly purified dendritic cell preparations can be obtained from these populations by sorting of fluorescence-labeled cells. Alternatively, dendritic cells can be generated from monocytes by culture with the appropriate cytokines, as described here. In addition, a negative selection approach is provided that may be employed to generate cell preparations that have been depleted of dendritic cells to be used for comparison in functional studies.

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Published In

Curr Protoc Immunol

DOI

EISSN

1934-368X

Publication Date

November 2012

Volume

Chapter 7

Start / End Page

7.32.1 / 7.32.23

Location

United States

Related Subject Headings

  • Lymphocyte Activation
  • Leukocytes, Mononuclear
  • Immunology
  • Humans
  • Flow Cytometry
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Cell Separation
  • Cell Proliferation
 

Citation

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Nair, S., Archer, G. E., & Tedder, T. F. (2012). Isolation and generation of human dendritic cells. Curr Protoc Immunol, Chapter 7, 7.32.1-7.32.23. https://doi.org/10.1002/0471142735.im0732s99
Nair, Smita, Gerald E. Archer, and Thomas F. Tedder. “Isolation and generation of human dendritic cells.Curr Protoc Immunol Chapter 7 (November 2012): 7.32.1-7.32.23. https://doi.org/10.1002/0471142735.im0732s99.
Nair S, Archer GE, Tedder TF. Isolation and generation of human dendritic cells. Curr Protoc Immunol. 2012 Nov;Chapter 7:7.32.1-7.32.23.
Nair, Smita, et al. “Isolation and generation of human dendritic cells.Curr Protoc Immunol, vol. Chapter 7, Nov. 2012, pp. 7.32.1-7.32.23. Pubmed, doi:10.1002/0471142735.im0732s99.
Nair S, Archer GE, Tedder TF. Isolation and generation of human dendritic cells. Curr Protoc Immunol. 2012 Nov;Chapter 7:7.32.1-7.32.23.

Published In

Curr Protoc Immunol

DOI

EISSN

1934-368X

Publication Date

November 2012

Volume

Chapter 7

Start / End Page

7.32.1 / 7.32.23

Location

United States

Related Subject Headings

  • Lymphocyte Activation
  • Leukocytes, Mononuclear
  • Immunology
  • Humans
  • Flow Cytometry
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Cell Separation
  • Cell Proliferation