Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse.


Journal Article (Review)

The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.

Full Text

Cited Authors

  • Augustine, GJ; Morgan, JR; Villalba-Galea, CA; Jin, S; Prasad, K; Lafer, EM

Published Date

  • February 2006

Published In

Volume / Issue

  • 34 / Pt 1

Start / End Page

  • 68 - 72

PubMed ID

  • 16417485

Pubmed Central ID

  • 16417485

Electronic International Standard Serial Number (EISSN)

  • 1470-8752

International Standard Serial Number (ISSN)

  • 0300-5127

Digital Object Identifier (DOI)

  • 10.1042/BST0340068


  • eng