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Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro.

Publication ,  Journal Article
Peters, EB; Christoforou, N; Leong, KW; Truskey, GA
Published in: Tissue engineering. Part A
March 2013

Coculture of endothelial cells (ECs) and smooth muscle cells (SMCs) in vitro can yield confluent monolayers or EC networks. Factors influencing this transition are not known. In this study, we examined whether the spatial arrangement of EC-SMC cocultures affected EC migration, network morphology, and angiogenic protein secretion. Human umbilical cord blood-derived ECs (hCB-ECs) were grown in coculture with human aortic SMCs in either a mixed or lamellar spatial geometry and analyzed over a culture period of 12 days. The hCB-ECs cultured on SMCs in a mixed system had higher cell speeds, shorter persistence times, and lower random motility coefficients than ECs in a lamellar system. By day 12 of coculture, mixed systems demonstrated greater anastomoses and capillary loop formation than lamellar systems as evidenced by a higher number of branch points, angle of curvature between branch points, and percentage of imaged area covered by networks. The network morphology was more uniform in the mixed systems than the lamellar systems with fewer EC clusters present after several days in culture. Proliferation of hCB-ECs was higher for mixed cocultures during the first 24 h of coculture, and then declined dramatically suggesting that proliferation only contributed to network formation during the early stages of coculture. Proteome assay results show reduced solution levels, but no change in intracellular levels of angiogenic proteins in lamellar systems compared to mixed systems. These data suggest that mixing ECs and SMCs together favors the formation of EC networks to a greater extent than a lamellar arrangement in which ECs form a cell layer above a confluent, quiescent layer of SMCs.

Duke Scholars

Published In

Tissue engineering. Part A

DOI

EISSN

1937-335X

ISSN

1937-3341

Publication Date

March 2013

Volume

19

Issue

5-6

Start / End Page

697 / 706

Related Subject Headings

  • Transduction, Genetic
  • Tissue Engineering
  • Neovascularization, Physiologic
  • Myocytes, Smooth Muscle
  • Humans
  • Fetal Blood
  • Endothelial Cells
  • Coculture Techniques
  • Cell Movement
  • Cell Aggregation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Peters, E. B., Christoforou, N., Leong, K. W., & Truskey, G. A. (2013). Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro. Tissue Engineering. Part A, 19(5–6), 697–706. https://doi.org/10.1089/ten.tea.2011.0704
Peters, Erica B., Nicolas Christoforou, Kam W. Leong, and George A. Truskey. “Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro.Tissue Engineering. Part A 19, no. 5–6 (March 2013): 697–706. https://doi.org/10.1089/ten.tea.2011.0704.
Peters EB, Christoforou N, Leong KW, Truskey GA. Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro. Tissue engineering Part A. 2013 Mar;19(5–6):697–706.
Peters, Erica B., et al. “Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro.Tissue Engineering. Part A, vol. 19, no. 5–6, Mar. 2013, pp. 697–706. Epmc, doi:10.1089/ten.tea.2011.0704.
Peters EB, Christoforou N, Leong KW, Truskey GA. Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro. Tissue engineering Part A. 2013 Mar;19(5–6):697–706.

Published In

Tissue engineering. Part A

DOI

EISSN

1937-335X

ISSN

1937-3341

Publication Date

March 2013

Volume

19

Issue

5-6

Start / End Page

697 / 706

Related Subject Headings

  • Transduction, Genetic
  • Tissue Engineering
  • Neovascularization, Physiologic
  • Myocytes, Smooth Muscle
  • Humans
  • Fetal Blood
  • Endothelial Cells
  • Coculture Techniques
  • Cell Movement
  • Cell Aggregation