On ribosomal gene compensation in Drosophila.

Journal Article (Journal Article)

Recent studies indicate that genes from only one nucleolus organizer undergo replication during polytenization of salivary gland cells of X/Y flies. This report presents evidence that this is also true X/X polytene cells. Interstrain hybrids were constructed which contain X chromosomes with different rDNA hybridization patterns following digestion with Eco RI, and DNA from diploid or polytene tissue was analyzed using Southern transfers. DNA from diploid tissue of X/X hybrids shows rDNA patterns from both X chromosomes, while DNA from polytene tissue of hybrids shows a pattern characteristic of only one of the X chromosomes. The polytene pattern of the hybrid is independent of whether the dominant X chromosome was derived from the female or male parent. These results suggest that genes from only one nucleolus organizer undergo multiple rounds of replication in X/X polytene cells, and account for the finding that X/O and X/X polytene cells contain similar levels of rDNA without postulating compensation as a ribosomal gene rectification mechanism.

Full Text

Duke Authors

Cited Authors

  • Endow, SA

Published Date

  • November 1, 1980

Published In

Volume / Issue

  • 22 / 1 Pt 1

Start / End Page

  • 149 - 155

PubMed ID

  • 6775815

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(80)90163-4


  • eng

Conference Location

  • United States